Project plan :Build a Gel Electrophoresis Apparatus
1.A Gel Electrophoresis Apparatus industry standard is showed below:
2.I have tried to DIY a Gel Electrophoresis Apparatus as below:
3.Equipment and Materials:
(1)The DC supply needs: Leads, alligator clip (2);Carbon rod(and sheet copper);Batteries, 9V transistor (5, 7, or 9).
(2)The Gel Electrophoresis comb can be replaced by a ordinary comb with wide teeth, or DIY with some suitable materials.
(3)Making gel needs:Plastic containers;Preservative film;Agar;250mL Beaker;100 mL Graduated cylinder.
(4)Simulated electrophoresis experiment needs:Glycerol;Pipettes;Reaction plate;Stain: Hucker’s crystal violet, Methylene blue;Stirring rod.
4.The result of the expriment is:
We can see, because of the molecular weight of the stains are different, the the moving speed of the stains are not the same, and the different stains can be separated.
5.The deficienty of our experiment
This experiment takes a long time, 30 minutes before they get a better separation effect, this is
because the battery as the power supply, the voltage is not enough, so the separation is slow,
and the use of batteries, not environmentally friendly.
Our project plan
We will try to develop the DC supply, and make it more efficient. So that we can spend less time to get the better separetion effect.